Guava nexin assay
WebDec 5, 2011 · The Guava Nexin assay utilizes two stains (annexin V and 7-amino actinomycin D [7-AAD]). Annexin V-PE binds to PS on the cell surface of apoptotic cells and 7-AAD, the cell impermeant dye is an indicator of membrane structural integrity. 7-AAD is excluded from live, healthy and early apoptotic cells, but permeates late stage apoptotic … WebGuava Nexin Annexin V Staining Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - …
Guava nexin assay
Did you know?
WebJun 11, 2009 · Complement-dependent cytotoxicity (CDC) and antibody-dependent cellular cytotoxicity (ADCC) assays were performed using Daudi cells as described previously. 9 Blood specimens were collected under a protocol approved by the New England Institutional Review Board (Wellesley, MA), and informed consent was obtained in accordance with … WebNov 1, 2013 · The impact of geranylgeraniol and lovastatin (Lova) on the initiation of apoptosis in human DU145 prostate carcinoma cells. (a) DU145 cells were incubated with geranylgeraniol (0, 50, and 100 μmol/L) and lovastatin (10 μmol/L) for 24 h and then analyzed by flow cytometry using the Guava Nexin ™ Assay. Cells in the lower left …
WebApr 1, 2013 · Annexin V staining for detecting apoptotic cells (Guava assay) Phosphatidylserine externalization was detected using an Annexin V staining kit (Millipore) following the manufacturer's instructions. Annexin V–positive cells were detected using the Guava System and analyzed with the Guava Nexin software Module (Millipore). WebThe new Guava Nexin® reagent monitors induction of apoptosis quickly and easily, with minimal chance for contamination. Annexin V is a calcium-dependent phospholipid …
WebApr 30, 2009 · The Guava Nexin assay (part number 4500-0161) was done following the manufacturer's instructions. Briefly, 3 × 10 4 cells (50 μL) were added to a 150 μL staining solution containing 135 μL of apoptosis buffer, 10 μL Annexin V-PE, and 5 μL of 7-AAD. The cells were incubated in the dark at room temperature for 20 min. Samples (2,000 cells ... WebAug 18, 2024 · Apoptosis Analysis. The percentage of apoptotic cells was evaluated by the Guava Nexin Assay. Briefly, the percentage of live, apoptotic and necrotic cells was assessed using the Guava Nexin Reagent (containing 7-AAD and Annexin-V-PE) and analyzed by Guava Nexin software.
WebJun 20, 2008 · The new mix-and-read Guava Nexin assay relies on a two-dye strategy. The assay uses Annexin V-PE to detect the translocation of phosphatidylserine to the …
WebDescription Guava Nexin Reagent for Flow Cytometry - 100 tests Overview The Guava Nexin® Annexin V Assay offers a simple, reproducible, and reliable assessment of early apoptosis. The assay relies on the translocation of phosphatidyl serine (PS) to the outer surface of the cell membrane, an event often associated with the onset of apoptosis. ford vehicle information by vinWebDec 1, 2024 · Guava Nexin assay (4500-0450, Guava Technologies, CA, USA) was utilized for measuring HaCaT cell apoptosis as earlier described [22]. Briefly, cells were … ford vehicle inspection checklistWebDec 17, 2024 · The results obtained with the Guava Nexin assay showed similar behavior for all the substances analyzed except for 2C-B. In fact, 2C-B stimulated the apoptosis of TK6 cells only at the highest concentrations tested (25 and 35 µM). This fact is of crucial toxicological importance and has great repercussions in terms of genotoxicity, because it ... ford vehicle lease dealsWebThe Guava® Nexin Reagent offers a simple, reproducible, and sensitive assessment of early apoptosis. embedded tools使用WebDec 1, 2024 · Guava Nexin assay (4500-0450, Guava Technologies, CA, USA) was utilized for measuring HaCaT cell apoptosis as earlier described [22]. Briefly, cells were cultivated into 6-well plate and subjected to relevant treatment. embedded time series databaseWebAug 6, 2024 · Cell proliferation was measured using 5-bromo-2′-deoxyuridine (BrdU) incorporation assay. Cell apoptosis was assessed using Guava Nexin assay. Cell migration and invasion were determined using two-chamber migration (invasion) assay. Cell transfection was used to change the expression of miR-21. embedded toolsWebSep 16, 2013 · Guava Nexin Assay. The assay was performed following manufactory protocol (Millipore). Briefly, attached and suspended cells were all collected. Cells were suspended in 100 µL of medium and incubated with 100 µL of Guava Nexin Reagent in each well for 20 minutes at room temperature in the dark. Samples then were measured … ford vehicle inventory search