WebName of substance Proteinase K CAS No 39450-01-6 EC No 254-457-8 Index No 647-014-00-9 SECTION 4: First aid measures 4.1 Description of first aid measures General notes … WebApr 30, 2024 · Store RNase A and Proteinase K at -20°C. Add ethanol (≥ 95%) to the gDNA Wash Buffer concentrate as indicated on the bottle label. Set a thermal mixer (e.g. ThermoMixer ®) or, if not available, a heating block to 56°C for sample lysis. Set a heating block to 60°C. Preheat the appropriate volume of elution buffer to 60°C (35-100 μl per ...
Enhancement of thermal stability of proteinase K by …
WebProteinase K from the fungus Engyodontium album is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K remains active: • Over a wide pH … WebThe best way to determine optimal Proteinase K digestion conditions is to do a Proteinase K titration experiment and then hybridize with a probe known to bind to RNA in the tissue. Choose the Proteinase K digestion conditions that produce the highest hybridization signal with the least disruption of tissue or cellular morphology. carola kruse
Roche Proteinase K recombinant PCR Grade lyophilizate Roche
WebMay 3, 2024 · Proteinase K (PK) is a proteolytic enzyme that has been widely used in nucleic acid purification, leather production, environmental protection, and other industrial applications. However, this biocatalyst cannot tolerate high temperatures which has severely restricted its wider application. As reported in pr Web20 mg/ml, sterile, ready-to-use, for biochemistry and molecular biology⮞Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family. Pro... WebMar 25, 2015 · 2) You can just weigh out the amount that will give you the desired concentration (which depends on the volume of buffer you're making). Example: Again, you want to prepare 5 mL of 200 µg/mL proteinase K. The total mass you need is: 200 μ g / m L ⋅ 5 m L = 1000 μ g. So weigh out a milligram and bring it up to 5 mL. carola kremling